barnes maze apparatus san diego instruments  (San Diego Instruments)

Journal: JCI Insight

Article Title: Mapping SCA1 regional vulnerabilities reveals neural and skeletal muscle contributions to disease

doi: 10.1172/jci.insight.176057

Figure Lengend Snippet: ( A ) Organization of the mouse WT-Atxn1 (blue) and human ATXN1 (black) genes, with the only 2 exons encoding the ATXN1 protein indicated by boxes larger and darker than the noncoding exons. The size (kb) and location of the mouse genomic sequences (blue) replaced by the human genomic sequences (black) in the f-ATXN1 146Q allele are indicated. ( B ) The portion of the Atxn1 gene encompassing the 2 coding exons was replaced with an FRT-recombination recipient cassette in mouse ES cells; then, that cassette was replaced with the portion of the human ATXN1 genomic sequences syntenic to the deleted mouse sequence using FLP recombinase. ( C ) The inserted human sequences in the resulting ATXN1 146Q allele are flanked by LOX recombination sites, as shown. Mating mice with this allele to lines expressing CRE recombinase removes the human ATXN1 insertion, as shown. ( D ) Barnes maze performance at 24 weeks of age, using unpaired 2-tailed t test. ( E ) Mouse survival plotted as Kaplan-Meier curves with median lifespan labeled for each genotype. Log-rank (Mantel Cox) **** P < 0.0001 and Gehan-Breslow-Wilcoxon #### P < 0.0001. ( F ) Body weight measurements between 6 and 36 weeks of age, repeated-measures 2-way ANOVA with Geisser-Greenhouse correction and Šídák’s post hoc test. ( G ) Representative photographs of 42-week-old WT-Atxn1 2Q/2Q and f-ATXN1 146Q/2Q showing kyphosis. * P < 0.05, ** P < 0.01, and **** P < 0.0001.

Article Snippet: A Barnes maze apparatus (San Diego Instruments) was used to assess spatial learning and memory ( ).

Techniques: Genomic Sequencing, Sequencing, Expressing, Labeling

Journal: JCI Insight

Article Title: Mapping SCA1 regional vulnerabilities reveals neural and skeletal muscle contributions to disease

doi: 10.1172/jci.insight.176057

Figure Lengend Snippet: ( A ) Open-field performance at 12 weeks of age, using 1-way ANOVA Tukey’s post hoc test. ( B ) Barnes maze performance at 24 weeks of age, using 1-way ANOVA Tukey’s post hoc test. ( C ) Mouse survival plotted as Kaplan-Meier curves with median lifespan labeled for each genotype. Log-rank (Mantel Cox) **** P < 0.0001 and Gehan-Breslow-Wilcoxon #### P < 0.0001. ( D ) Body weight measurements between 7 and 48 weeks of age. Significant weight difference shown is between f-ATXN1 146Q/2Q and f-ATXN1 146Q/2Q ;Nestin-Cre at 38 weeks. Repeated-measures 2-way ANOVA Geisser-Greenhouse correction and Tukey’s post hoc test. ( E ) Hind limb clasping at 36 weeks. * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001.

Article Snippet: A Barnes maze apparatus (San Diego Instruments) was used to assess spatial learning and memory ( ).

Techniques: Labeling